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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
09/03/2020 |
Data da última atualização: |
20/04/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SIMAS, P. V. M; BASSETTO, C. C.; GIGLIOTI, R.; OKINO, C. H.; OLIVEIRA, H. N. de; OLIVEIRA, M. C. de S. |
Afiliação: |
Paulo Vitor Marques Simas, UNESP; César Cristiano Bassetto, UNESP; Rodrigo Giglioti, Instituto de Zootecnia; CINTIA HIROMI OKINO, CPPSE; Henrique Nunes de Oliveira, UNESP; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
Título: |
Use of molecular markers can help to understand the genetic diversity of Babesia bovis. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Infection, Genetics and Evolution, v. 79, 2020. 104161. |
Páginas: |
9 |
ISSN: |
1567-1348 |
DOI: |
https://doi.org/10.1016/j.meegid.2019.104161 |
Idioma: |
Inglês |
Conteúdo: |
Cattle babesiosis is a tick-borne disease responsible for significant losses for the livestock industries in tropical areas of the world. These piroplasms are under constant control of the host immune system, which lead to a strong selective pressure for arising more virulent or attenuated phenotypes. Aiming to better understand the most critical genetic modifications in Babesia bovis genome, related to virulence, an in silico analysis was performed using DNA sequences from GenBank. Fourteen genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80 (or Bb-1), 18S rRNA, acs-1, ama-1, ?-tub, cp-2, p0, rap-1a) related to parasite infection and immunogenicity and ITS region were selected for alignment and comparison of several isolates of Babesia bovis from different geographic regions around the world. Among the 15 genes selected for the study of diversity, only 7 genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80) and the ITS region presented sufficient genetic variation for the studies of phylogeny. Despite this genetic diversity observed into groups, there was not sufficient information available to associate molecular markers with virulence of isolates. However, some genetic groups no were correlated with geographic region what could indicate some typical evolutionary characteristics in the relation between parasite-host. Further studies using these genes in herds presenting diverse clinical conditions are required. The better understanding of evolutionary mechanisms of the parasite may contribute to improve prophylactic and therapeutic measures. In this way, we suggest that genes used in our study are potential markers of virulence and attenuation and have to be analyzed with the use of sequences from animals that present clinical signs of babesiosis and asymptomatic carriers. MenosCattle babesiosis is a tick-borne disease responsible for significant losses for the livestock industries in tropical areas of the world. These piroplasms are under constant control of the host immune system, which lead to a strong selective pressure for arising more virulent or attenuated phenotypes. Aiming to better understand the most critical genetic modifications in Babesia bovis genome, related to virulence, an in silico analysis was performed using DNA sequences from GenBank. Fourteen genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80 (or Bb-1), 18S rRNA, acs-1, ama-1, ?-tub, cp-2, p0, rap-1a) related to parasite infection and immunogenicity and ITS region were selected for alignment and comparison of several isolates of Babesia bovis from different geographic regions around the world. Among the 15 genes selected for the study of diversity, only 7 genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80) and the ITS region presented sufficient genetic variation for the studies of phylogeny. Despite this genetic diversity observed into groups, there was not sufficient information available to associate molecular markers with virulence of isolates. However, some genetic groups no were correlated with geographic region what could indicate some typical evolutionary characteristics in the relation between parasite-host. Further studies using these genes in herds presenting diverse clinical conditions are required. The better understanding of evolutionary mechanisms of th... Mostrar Tudo |
Palavras-Chave: |
Attenuation; Genetic diversity; Molecular markers. |
Thesagro: |
Babesia Bovis. |
Thesaurus Nal: |
Virulence. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/211610/1/Use-of-molecular-markers-can-help-to-understand-the-2020-Infection-Genetics.pdf
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Marc: |
LEADER 02625naa a2200277 a 4500 001 2121033 005 2020-04-20 008 2020 bl uuuu u00u1 u #d 022 $a1567-1348 024 7 $ahttps://doi.org/10.1016/j.meegid.2019.104161$2DOI 100 1 $aSIMAS, P. V. M 245 $aUse of molecular markers can help to understand the genetic diversity of Babesia bovis.$h[electronic resource] 260 $c2020 300 $a9 520 $aCattle babesiosis is a tick-borne disease responsible for significant losses for the livestock industries in tropical areas of the world. These piroplasms are under constant control of the host immune system, which lead to a strong selective pressure for arising more virulent or attenuated phenotypes. Aiming to better understand the most critical genetic modifications in Babesia bovis genome, related to virulence, an in silico analysis was performed using DNA sequences from GenBank. Fourteen genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80 (or Bb-1), 18S rRNA, acs-1, ama-1, ?-tub, cp-2, p0, rap-1a) related to parasite infection and immunogenicity and ITS region were selected for alignment and comparison of several isolates of Babesia bovis from different geographic regions around the world. Among the 15 genes selected for the study of diversity, only 7 genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80) and the ITS region presented sufficient genetic variation for the studies of phylogeny. Despite this genetic diversity observed into groups, there was not sufficient information available to associate molecular markers with virulence of isolates. However, some genetic groups no were correlated with geographic region what could indicate some typical evolutionary characteristics in the relation between parasite-host. Further studies using these genes in herds presenting diverse clinical conditions are required. The better understanding of evolutionary mechanisms of the parasite may contribute to improve prophylactic and therapeutic measures. In this way, we suggest that genes used in our study are potential markers of virulence and attenuation and have to be analyzed with the use of sequences from animals that present clinical signs of babesiosis and asymptomatic carriers. 650 $aVirulence 650 $aBabesia Bovis 653 $aAttenuation 653 $aGenetic diversity 653 $aMolecular markers 700 1 $aBASSETTO, C. C. 700 1 $aGIGLIOTI, R. 700 1 $aOKINO, C. H. 700 1 $aOLIVEIRA, H. N. de 700 1 $aOLIVEIRA, M. C. de S. 773 $tInfection, Genetics and Evolution$gv. 79, 2020. 104161.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
10/05/2017 |
Data da última atualização: |
25/07/2018 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
TEIXEIRA, F. F.; SABATO, E. de O. |
Afiliação: |
FLAVIA FRANCA TEIXEIRA, CNPMS; ELIZABETH DE OLIVEIRA SABATO, CNPMS. |
Título: |
Resistência do milho aos enfezamentos causados por molicutes. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
In: OLIVEIRA, C. M. de; SABATO, E. de O. (Ed.). Doenças em milho: insetos-vetores, molicutes e vírus. Brasília, DF: Embrapa, 2017. cap. 11, p. 181-188. |
Idioma: |
Português |
Notas: |
Texto em português e inglês. Título equivalente: Corn resistance to stunting caused by mollicutes. |
Thesagro: |
Controle genético; Doença de planta; Enfezamento; Resistência genética. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 00735naa a2200181 a 4500 001 2069478 005 2018-07-25 008 2017 bl uuuu u00u1 u #d 100 1 $aTEIXEIRA, F. F. 245 $aResistência do milho aos enfezamentos causados por molicutes.$h[electronic resource] 260 $c2017 500 $aTexto em português e inglês. Título equivalente: Corn resistance to stunting caused by mollicutes. 650 $aControle genético 650 $aDoença de planta 650 $aEnfezamento 650 $aResistência genética 700 1 $aSABATO, E. de O. 773 $tIn: OLIVEIRA, C. M. de; SABATO, E. de O. (Ed.). Doenças em milho: insetos-vetores, molicutes e vírus. Brasília, DF: Embrapa, 2017. cap. 11, p. 181-188.
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